Sub-cellular internalization and organ specific oral elivery of PABA nanoparticles by side chain variation
نویسندگان
چکیده
BACKGROUND Organic nanomaterials having specific biological properties play important roles in in vivo delivery and clearance from the live cells. To develop orally deliverable nanomaterials for different biological applications, we have synthesized several fluorescently labelled, self-assembled PABA nanoparticles using possible acid side chain combinations and tested against insect and human cell lines and in vivo animal model. Flurophores attached to nanostructures help in rapid in vivo screening and tracking through complex tissues. The sub-cellular internalization mechanism of the conjugates was determined. A set of physio-chemical parameters of engineered nanoskeletons were also defined that is critical for preferred uptake in multiple organs of live Drosophila. RESULTS The variability of side chains alter size, shape and surface texture of each nanomaterial that lead to differential uptake in human and insect cells and to different internal organs in live Drosophila via energy dependent endocytosis. Our results showed that physical and chemical properties of C-11 and C-16 acid chain are best fitted for delivery to complex organs in Drosophila. However a distinct difference in uptake of same nanoparticle in human and insect cells postulated that different host cell physiology plays a critical role in the uptake mechanism. CONCLUSIONS The physical and chemical properties of the nanoparticle produced by variation in the acid side chains that modify size and shape of engineered nanostructure and their interplay with host cell physiology might be the major criteria for their differential uptake to different internal organs.
منابع مشابه
PABA Nanoparticles as Effective Delivery Agents of siRNAs into Biological Systems
Organic nanomaterials hold promises as new-age drug delivery vehicles in vivo and in live cells. Authors have successfully synthesised several fluorescently labelled, self-assembled PABA (p-aminobenzoic acid) nanoparticles using various acid side chain combinations. p-aminobenzoic acid (PABA), a structural moiety of many commercial drugs, is selfassembled with linker alkyl side chains to form t...
متن کاملHemolytic Anemia and Other Side Effects of Para-amino Benzoic Acid in an 8-Year-Old Girl
Background: Para-amino benzoic acid (PABA) is an important ingredient used as a structure moiety in drugs with wide range of therapeutic uses; however, its safety and possible side effects in young children have not been determined. Case Presentation: An 8-year-old girl was admitted to the emergency department for paleness, jaundice, abdominal pain and nausea associated with Hemoglubin (Hb) =4...
متن کاملParameters and characteristics governing cellular internalization and trans-barrier trafficking of nanostructures
Cellular internalization and trans-barrier transport of nanoparticles can be manipulated on the basis of the physicochemical and mechanical characteristics of nanoparticles. Research has shown that these factors significantly influence the uptake of nanoparticles. Dictating these characteristics allows for the control of the rate and extent of cellular uptake, as well as delivering the drug-loa...
متن کاملA Comprehensive Mathematical Model for Designing an Organ Transplant Supply Chain Network under Uncertainty
One of the most important issues in area of health and hygiene is location-allocation of organ harvesting centers and transplant centers according to coordination between supply and demand. In this paper, a mathematical model is presented for location-allocation of organ harvesting centers and transplant centers. The proposed model does not only minimize the present value of the total system co...
متن کاملMesoporous Silica Nanoparticles as a Nanocarrier for Delivery of Vitamin C
Background: In the last decades, mesoporous silica nanoparticles (MSN) are improved for drug delivery, imaging, and biomedical applications due to their special properties such as large surface area, high drug loading capacity, tunable pore size, and modification of surface area by functional groups. Objectives: The aim of this study was to evaluate MSNs as carriers for oral colon-specific and...
متن کامل